Composite

Part:BBa_K2449024

Designed by: Malte Skovsager Andersen   Group: iGEM17_SDU-Denmark   (2017-10-23)


cenA-hlyA and cex-hlyA regulated by penI

This part is coding for cellulases endoglucanse and exoglucanase, which is able to breakdown cellulose to cellobiose. The hlyA-tags make the enzymes secreteable with the alpha-hemolysin secretion system

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 408
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 260
    Illegal BamHI site found at 1470
    Illegal BamHI site found at 2275
    Illegal BamHI site found at 3224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 300
    Illegal AgeI site found at 396
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 108


[edit]
Categories
Parameters
None